Tissue clearing of kidney, heart and liver to enable 3D organ imaging
Thesis project: 30 credits
Student location: AstraZeneca, Gothenburg, Sweden
Start: January 2022
Do you have experience of and interest in working with histology? Would you like to develop tissue clearing protocols for whole organ imaging ? Then this Master thesis project at AstraZeneca might be of interest to you!
For this project, you will join Cell and Molecular Pharmacology (CMP) team that delivers key data within histology, imaging, biomarker, FACS and bioassay automation to Cardiovascular, Renal and Metabolism (CVRM) disease areas. Within the histology group we are at the scientific forefront of latest histology technique and image analyses.
State- of- Art tissue clearing combined with fluorescent labelling (tissue structures/compounds/antibodies/proteins/cells) and imaging can provide valuable visualization of 3D structure together with target distribution through whole organ.
3D organ imaging is hampered due to low light penetration through the tissue. Major cause of low tissue transparency is light scattering caused by organ components such as lipids, pigments and collagen fibers. By using optical clearing agents (OCAs) these disturbing components are removed from the tissue, leading to improvement of light penetration and increasing tissue transparency and enabling whole organ imaging.
- The goal of thesis is to develop tissue clearing protocol to achieve tissue transparency while preserving organ morphology and fluorescent labelling for kidney, heart and liver.
As master student you will be a part of our histology team and learn about tissue fixation, processing, labelling, and imaging.
We look forward working with you!
- Well-organized and focused mindset
- Strong oral and written communication skills.
- Desire to learn and try new things.
Randstad Life Sciences is cooperating with AstraZeneca in this recruitment process. We only handle applications sent through Randstad’s website.
All chosen candidates need to pass a background check and an alcohol and drug test before starting the project. Deadline for application: 2021-10-29
For more information: Veronika Knudsen firstname.lastname@example.org
or Kerstin Karlsson email@example.com
At AstraZeneca, we turn ideas into life changing medicines and strive to continuously meet the unmet needs of patients worldwide. Working here means being entrepreneurial, thinking big and working together to make the impossible a reality. If you are swift to action, confident to lead, willing to collaborate, and curious about what science can do, then you’re our kind of person.
Our Gothenburg site is one of AstraZeneca's three strategic science centers. We thrive in a multinational environment working cross-functionally across the globe with AstraZeneca colleagues as well as academic and industry partners. Our way of life is to foster a working environment that nurtures collaboration, openness and innovation. Therefore, we have created space for meetings, socializing and relaxation, where spontaneous meetings can give birth to new innovations. The unexpected ideas or thoughts that can come from a chat over something as simple as a cup of coffee or a stroll on our “walk and talk” meeting trail. AstraZeneca is an equal opportunity employer. AstraZeneca will consider all qualified applicants for employment without discrimination on grounds of disability, sex or sexual orientation, pregnancy or maternity leave status, race or national or ethnic origin, age, religion or belief, gender identity or re-assignment, marital or civil partnership status, protected veteran status (if applicable) or any other characteristic protected by law. AstraZeneca only employs individuals with the right to work in the country/ies where the role is advertised.