Thesis work: 30 credits/spring 2020
Novel 3D liver cell cultures in healthy and disease - model development and project application
Do you want to learn how to culture liver cells in 3D and apply them in disease models? Then this project might be of interest for you!
3D cultures of cells have emerged as a new technology to culture cells in a more in vivo like environment, for prolonged time. At Early CVRM DMPK we are working with 3D cultures of liver cells, both as spheroids of hepatocytes only and co-cultures of hepatocytes with other liver cell types. We have also developed a steatotic liver model based on these hepatic spheroids.
This is a thesis position in this fast-moving field, to evaluate the DMPK properties of the steatotic 3D cell cultures and for further development into a NASH disease model, which is a highly interesting model for CVRM. The student joins a team regularly working with static liver spheroids, applying them to address different project issues.
Research and Early Development, Cardiovascular Renal and Metabolism
Early CVRM is one of the three main therapeutic research areas within AstraZeneca which deliver candidate drugs into late-stage clinical development. Early CVRM is a global function with research units in Gaithersburg, US, Cambridge, UK and Gothenburg, Sweden; the DMPK department is based in AstraZeneca’s world-class vibrant R&D center, located in Gothenburg. DMPK interacts with other functions in supporting Early CVRM projects all along the value chain, from target selection all the way to market launch and Life cycle management.
As thesis worker you will be part of a team at the forefront of DMPK science focusing on metabolism, disposition, and analysis of small molecule, peptide and nucleotide drug candidates. You will execute experiments and provide significant input into one of our prioritised method development areas.
Throughout the placement, the student will work with 3D cultures of primary cells and can learn different analytical techniques e.g. immunohistochemistry, RT-PCR, ELISA, and LC-MSMS. It's integral part of this placement to discuss hypotheses, develop study designs and evaluate data. The work will focus on the thesis assignment, but also gives opportunity to work in the team to acquire a variety of in vitro ADME skills that are relevant to characterise the spheroids. The student will work in a collaborative environment, interacting with people from multiple disciplines within drug discovery.
- Education in Biology, Biotechnology, Biomedical Sciences, Biochemistry, or Pharmacy
- A strong interest in multidisciplinary biomedical research
- Experience in cell cultures and sterile work
- Experience with at least one of the analytical techniques applied in the project (immunohistochemistry, RT-PCR, ELISA, and LC-MSMS)
Deadline for application: 2019-11-17, selection and interviews will be ongoing. The position may be filled before the last day of application, therefore, apply as soon as possible.
For more information: Kerstin Karlsson email@example.com or Eleonor Ehrman +4673-343 41 09.
At AstraZeneca we turn ideas into life changing medicines and strive to continuously meet the unmet needs of patients worldwide. Working here means being entrepreneurial, thinking big and working together to make the impossible a reality. If you are swift to action, confident to lead, willing to collaborate, and curious about what science can do, then you’re our kind of person.
AstraZeneca is an equal opportunity employer. AstraZeneca will consider all qualified applicants for employment without discrimination on grounds of disability, sex or sexual orientation, pregnancy or maternity leave status, race or national or ethnic origin, age, religion or belief, gender identity or re-assignment, marital or civil partnership status, protected veteran status (if applicable) or any other characteristic protected by law. AstraZeneca only employs individuals with the right to work in the country/ies where the role is advertised.